UnderstandingCampylobacter coliisolates from the Vietnamese meat production network; a pilot study

Abstract

AbstractChanging farming practices and the associated increase in the use of antibiotics are amongst the main drivers shaping the global increase of Campylobacter infections. The effects farming practices have onCampylobacterspecies, need to be studied at the global scale, particularly in emerging middle-income countries, where the demand for low-cost poultry meat is rising. WhileC. jejunicauses the majority of poultry associated diarrhoea,C. colicauses a significant amount of disease but are relatively understudied. In this study we characterised sevenC. colistrains isolated from poultry farms and markets in Hanoi, Vietnam. Comprehensive data sets of bacterial Whole-Genome Sequencing; and phenotypic assays, such as, growth, motility, antimicrobial resistant testing along with virulence testing were performed to reveal the genetic relatedness and pathophysiological characteristics of sevenC. colistrains. Six isolates were classified as multi-drug resistant, with all isolates resistant to ciprofloxacin, nalidixic acid and tetracycline, but susceptible to phenicols. All isolates had similar growth rates, while five were hyper-motile. Lethality of the isolates towards a tractable host-model system, larvae of the greater wax mothGalleria mellonella, often used to determineCampylobactervirulence was demonstrated for the first time forC. coli. Multilocus sequence typing data correlates with North American, European, and Asian isolates from patients suffering from gastroenteritis, emphasising the global spread of these strains. This work demonstrates thatC. coli, with high levels of antimicrobial resistance, is an understudied global threat.Data summaryGenBank database with accession numbers JAKGTW000000000, JAKGTV000000000, JAKGTS000000000, JAKGTU000000000, JAKGTT000000000, JAKGTR000000000 and CP091310https://www.ncbi.nlm.nih.gov/nuccore/JAKGTW000000000https://www.ncbi.nlm.nih.gov/nuccore/JAKGTV000000000https://www.ncbi.nlm.nih.gov/nuccore/JAKGTS000000000https://www.ncbi.nlm.nih.gov/nuccore/JAKGTU000000000https://www.ncbi.nlm.nih.gov/nuccore/JAKGTT000000000https://www.ncbi.nlm.nih.gov/nuccore/JAKGTR000000000https://www.ncbi.nlm.nih.gov/nuccore/CP091310.1The authors confirm all supporting data, code and protocols have been provided within the article or through supplementary data files.