Affiliation:
1. Department of Biology. College of Sciences University of Mosul, Iraq
2. Al-Salam Teaching Hospital. Nineveh Health Directorate, Iraq
Abstract
The oxidative stress (OS) and sperm DNA fragmentation have been associated with sub-infertility in males and infertility due to a lack of means of protection and defense (antioxidants) for spermatozoa and an increase in reactive oxygen spices (ROS) related to wrong lifestyle (cigarettes and hookah) or what also known as Shisha. The study included (90) men between the ages of (23-40) years, (60), infertile men who smoked cigarettes and hookahs. They were divided into two groups, the first oligozoospermia and the second asthenozoospermia, according to the guidelines of the World Health Organization for seminal fluid analysis (SFA), 5 th edition, WHO(2010). Each group included (30) smoking infertile males, with a control group included (30) non-smoking fertile males. The study aimed to estimate the concentration level of Ascorbic Acid (AA) in the seminal plasma of two groups of infertile men smoking cigarettes and hookahs using enzyme-linked immunosorbent assay (ELISA) method, with calculating the concentration of Malondialdehyde (MDA) as well as estimating the percentage of sperm DNA fragmentation (SDF) using sperm chromatin dispersion (SCD), Halo view. Compared with healthy fertile men of non-smokers of cigarettes and hookahs as one of the biomarkers of oxidative stress in infertile men in Nineveh Governorate-Iraq. The results indicated that there were significant differences at the probability level (p<0.01) for the studied parameters (AA, MDA, SDF) compared to the control group (fertile men). The results showed an increase in the percentage of sperm DNA fragmentation with increasing the concentration of MDA in seminal plasma and decreasing the concentration of AA. Cigarette and hookah smoking increased the generation of ROS with a decrease in the water-soluble non-enzymatic antioxidant Ascorbic acid (AA) and thus increased the oxidative processes of the plasma membranes of spermatozoa and the occurrence of oxidative stress and impaired fertility and caused infertility. Keywords: male infertility, oxidative stress, sperm DNA fragmentation, Ascorbic acid, MDA. Bionatura http://dx.doi.org/10.21931/RB/CSS/2023
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
Reference87 articles.
1. 1. Sengupta, p.; Dutta, S; Krajewska - kulak, E. The Disappearing Sperm: Analysis of Reports published
2. between 1980 and 2015. Am J Mens Health 2017, 11 (4): 1279-1304 2
3. 2. Levine, H. Jorgensen, N.; Martine- Andrade, A.; Mendiola, J.; weksler – Derri, D.; Swan, S.H.
4. Temporal Trends in sperm count: a systematic review and meta-regression analysis-Human reproduction update 2017, 23 (6); 646-659.
5. 3. Jungwirth , A.; Diemer. T.; Dahle, R., Giwereman, A.; Kopa , Z.; Krak, S. C.; Tournaye, H. (2012).
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献