Response surface methodology-based optimization studies about bioethanol production by Candida boidinii from pumpkin residues
Author:
Demiray Ekin1ORCID, Karatay Sevgi Ertuğrul2ORCID, Dönmez Gönül2ORCID
Affiliation:
1. ANKARA YILDIRIM BEYAZIT UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL, DEPARTMENT OF MEDICAL SERVICES AND TECHNIQUES, MEDICAL LABORATORY TECHNIQUES PR. 2. ANKARA UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY, BIOLOGY PR.
Abstract
For sustainable bioethanol production, the investigation of novel fermentative microorganisms and feedstocks is crucial. In this context, the goals of the current study are suggesting pumpkin residues as new raw material for bioethanol production and investigating the fermentative capacity of the Candida boidinii, which is a newly isolated yeast from sugar factory wastes. Response surface methodology was used to determine the effect of enzyme (cellulase and hemicellulase) concentration and enzymatic hydrolysis time. The maximum bioethanol concentration was 29.19 g/L when fermentation parameters were optimized. However, it is revealed that enzymatic hydrolysis and hydrolysis duration (48-72 h) have significant effects on reducing sugar concentration. The highest reducing sugar was 108.86 g/L when the 20% initial pumpkin residue was hydrolyzed at 37.5 FPU/g substrate cellulase and 37.5 U/mL hemicellulase at the end of 72 h. Under these optimized conditions, the bioethanol production of C. boidinii increased by 22.91% and reached 35.88 g/L. This study shows pumpkin residues are promising feedstocks and C. boidinii is a suitable microorganism for efficient bioethanol production.
Funder
This work was supported by Research Foundation of Ankara University
Publisher
Field Crops Central Research Institute
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