A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide-Reference-Cited by-同舟云学术

A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide

Published:1998-06 Issue:6 Volume:36 Page:1801-1804
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Desmarchelier Patricia M.¹,Bilge Sima S.²,Fegan Narelle¹,Mills Leanne¹,Vary James C.²,Tarr Phillip I.²

Affiliation:

1. Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia,1and

2. Children’s Hospital and Medical Center and the University of Washington, Seattle, Washington2

Abstract

ABSTRACT A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in raw milk following enrichment.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.6.1801-1804.1998

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