Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number-Reference-Cited by-全球学者库

Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number

Published:2020-07-23 Issue:8 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chen Hailong¹,Wu Rui¹,Xing Yuan²,Du Quanli¹,Xue Zerun¹,Xi Yanli¹,Yang Yujie¹,Deng Yangni¹,Han Yuewen¹,Li Kaixin¹,Luan Yang¹,Zhang Yalan¹,Wei Xiaoguang¹,Yu Tongtong¹,Li Hao¹,Zhu Lingxiang³,Su Shisheng⁴,Lian Hao⁵,Lu Linping⁵,Tan Chianru⁴,Zheng Haichao¹,Chen Baozhong¹,Yu Pengbo⁶,Guo Yong⁴,Ma Chaofeng¹^ORCID

Affiliation:

1. Xi’an Center for Disease Control and Prevention, Xi’an, Shaanxi, China

2. Xi’an Medical University, Xi’an, Shaanxi, China

3. National Research Institute for Family Planning, Beijing, China

4. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China

5. TargetingOne Corporation, Beijing, China

6. Shaanxi Center for Disease Control and Prevention, Xi’an, Shaanxi, China

Abstract

The outbreak of coronavirus disease 2019 (COVID-19) has spread across the world and was characterized as a pandemic. To protect medical laboratory personnel from infection, most laboratories inactivate the virus causing COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in clinical samples before testing. However, the effect of inactivation on the detection results remains unknown. Here, we used a digital PCR assay to determine the absolute SARS-CoV-2 RNA copy number in 63 nasopharyngeal swab samples and assess the effect of inactivation methods on viral RNA copy number.

Funder

Xi'an Science and Technology Bureau

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00958-20

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