Multicenter Evaluation of the Xpert Carba-R Assay for Detection and Identification of Carbapenemase Genes in Sputum Specimens

Author:

Cai Zhen1,Tao Jia2,Jia Tianye3,Fu Hongyu4,Zhang Xin56,Zhao Mei2,Du Hong4,Yu Hua5,Shan Bin7,Huang Bin8,Chen Liang910,Tang Yi-Wei61112ORCID,Jia Wei2,Qu Fen13

Affiliation:

1. China Aviation General Hospital of China Medical University, Beijing, China

2. Center of Medical Laboratory, the General Hospital of Ningxia Medical University, Yinchuan, China

3. The Center of Clinical Diagnosis Laboratory, 302 Hospital of PLA, Beijing, China

4. Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China

5. Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China

6. Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA

7. Department of Laboratory Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China

8. Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China

9. Hackensack-Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA

10. Hackensack Meridian School of Medicine, Seton Hall University, Nutley, New Jersey, USA

11. Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York, USA

12. Cepheid, Shanghai, China

Abstract

Rapid diagnosis of infections caused by carbapenem-resistant Enterobacteriaceae (CRE) is crucial for proper treatment and infection control. The Xpert Carba-R assay is a qualitative multiplex real-time PCR method that qualitatively detects and differentiates five common carbapenemase genes ( bla KPC , bla NDM , bla VIM , bla OXA-48 , and bla IMP ) directly from rectal swabs or purified colonies within approximately 1 h.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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