Interaction of the Echinocandin Caspofungin with Amphotericin B or Voriconazole against Aspergillus BiofilmsIn Vitro

Abstract

ABSTRACTAspergillusbiofilms were prepared from 22 strains ofAspergillusspp. via a 96-well plate-based method. Using a broth microdilution checkerboard technique with the XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] colorimetric assay, we demonstrated a synergistic antifungal activity against 18 of 22Aspergillusbiofilm strains with a combination of caspofungin and amphotericin B and against 13 of 22 strains with a combination of caspofungin and voriconazole. We did not observe antagonism.