Ectopic BH3-Only Protein Bim Associates with Hsp70 to Regulate Yeast Mitophagy
-
Published:2023-10
Issue:1
Volume:512
Page:292-299
-
ISSN:1607-6729
-
Container-title:Doklady Biochemistry and Biophysics
-
language:en
-
Short-container-title:Dokl Biochem Biophys
Author:
Yuan Linjie,Wang Yuying,Margulis B. A.,Song Ting,Wang Ziqian,Zhang Zhichao
Abstract
Abstract
Mitophagy, a form of selective autophagy, plays an essential role to maintain a population of healthy and functional mitochondria for normal cellular metabolism. Acting mainly as one of the B-cell lymphoma 2 (Bcl-2) family pro-apoptotic members, Bim (also known as BCL2L11) was identified to be a co-chaperone of Hsp70 to promote mitophagy in mammalian cells. Herein, with the help of a specific Hsp70/Bim disruptor and Om45-GFP processing assay, we illustrated that ectopic BimEL is able to promote mitophagy through Hsp70/Bim interaction in yeast, where Bax/Bak is absent. The Hsp70/Bim-mediated mitophagy is conserved in eukaryotes, from yeast to humans.
Publisher
Pleiades Publishing Ltd
Subject
General Chemistry,Biochemistry,General Medicine,Biophysics
Reference31 articles.
1. Hirota, Y., Kang, D., and Kanki, T., The physiological role of mitophagy: New insights into phosphorylation events, Int. J. Cell Biol., 2012, vol. 2012, pp. 1–8. 2. Li, W., He, P., Huang, Y., Li, Y.F., Lu, J., Li, M., Kurihara, H., Luo, Z., Meng, T., Onishi, M., et al., Selective autophagy of intracellular organelles: recent research advances, Theranostics, 2020, vol. 11, pp. 222–256. 3. Klionsky, D.J., The molecular machinery of autophagy: unanswered questions, J. Cell Sci., 2005, vol. 118, pp. 7–18. 4. Chen, Y.Q., and Klionsky, D.J., The regulation of autophagy—unanswered questions, J. Cell Sci., 2011, vol. 124, pp. 161–170. 5. Das, C.K., Mandal, M., and Kögel, D., Pro-survival autophagy and cancer cell resistance to therapy, Cancer Metastasis Rev., 2018, vol. 37, pp. 749–766.
|
|