Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization-Reference-Cited by-同舟云学术

Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization

Published:2016-09-15 Issue:11 Volume:128 Page:1465-1474
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

Wang Leo D.¹²³^ORCID,Ficarro Scott B.⁴⁵,Hutchinson John N.⁶,Csepanyi-Komi Roland⁷^ORCID,Nguyen Phi T.¹²,Wisniewski Eva⁷,Sullivan Jessica¹²,Hofmann Oliver⁶^ORCID,Ligeti Erzsebet⁷^ORCID,Marto Jarrod A.⁴⁵,Wagers Amy J.¹²⁸

Affiliation:

1. Joslin Diabetes Center, Boston, MA;

2. Harvard Stem Cell Institute, Harvard University, Cambridge, MA;

3. Dana-Farber Boston Children’s Cancer and Blood Disorders Center, Boston, MA;

4. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA;

5. Department of Cancer Biology and Blais Proteomics Center, Dana-Farber Cancer Institute, Boston, MA;

6. Department of Biostatistics, Harvard T. H. Chan School of Public Health, Harvard University, Boston, MA;

7. Department of Physiology, Semmelweis University, Budapest, Hungary; and

8. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA

Abstract

Key Points Combining flow cytometry and high-performance mass spectrometry enables phosphoproteomic analysis of rare blood cell populations. ARHGAP25 dephosphorylation augments activity and promotes blood stem and progenitor cell mobilization by enhancing CXCL12 and Rac signaling.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/128/11/1465/1395430/1465.pdf

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