Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

Published:2020-07-10 Issue:10 Volume:5 Page:1299-1305
ISSN:2058-5276
Container-title:Nature Microbiology
language:en
Short-container-title:Nat Microbiol

Author:

Vogels Chantal B. F.^ORCID,Brito Anderson F.^ORCID,Wyllie Anne L.,Fauver Joseph R.^ORCID,Ott Isabel M.,Kalinich Chaney C.^ORCID,Petrone Mary E.,Casanovas-Massana Arnau^ORCID,Catherine Muenker M.,Moore Adam J.,Klein Jonathan,Lu Peiwen^ORCID,Lu-Culligan Alice^ORCID,Jiang Xiaodong,Kim Daniel J.,Kudo Eriko,Mao Tianyang^ORCID,Moriyama Miyu,Oh Ji Eun,Park Annsea,Silva Julio,Song Eric^ORCID,Takahashi Takehiro,Taura Manabu,Tokuyama Maria,Venkataraman Arvind,Weizman Orr-El,Wong Patrick,Yang Yexin,Cheemarla Nagarjuna R.^ORCID,White Elizabeth B.^ORCID,Lapidus Sarah,Earnest Rebecca,Geng Bertie^ORCID,Vijayakumar Pavithra,Odio Camila,Fournier John,Bermejo Santos,Farhadian Shelli,Dela Cruz Charles S.,Iwasaki Akiko^ORCID,Ko Albert I.,Landry Marie L.,Foxman Ellen F.^ORCID,Grubaugh Nathan D.^ORCID

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Microbiology (medical),Genetics,Applied Microbiology and Biotechnology,Immunology,Microbiology

Link

http://www.nature.com/articles/s41564-020-0761-6.pdf

Reference18 articles.

1. Gorbalenya, A. E. et al. Severe acute respiratory syndrome-related coronavirus: the species and its viruses – a statement of the Coronavirus Study Group. Preprint at https://doi.org/10.1101/2020.02.07.937862 (2020).

2. Wu, F. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020).

3. Zhou, P. et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579, 270–273 (2020).

4. Chu, D. K. W. et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. Clin. Chem. 66, 549–555 (2020).

5. Corman, V. M. et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT–PCR. Euro Surveill. 25, 2000045 (2020).

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