MicroRNA-512-5p Derived from Bone Marrow Mesenchymal Stem Cells Suppresses Metastasis of Glioma Cells

Abstract

Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) are enriched in microRNA-512-5p (miR-512-5p) expression and can inhibit glioma cell growth, but the mechanism is not clear. This study assesses how BMSC-miR-512-5p functions in glioma cells to provide evidence for treatment of glioma. Exosomes were collected from BMSCs to measure miR-512-5p level. Glioma cells SHG-44 were transfected with BMSC-miR-512-5p and KLF15 inhibitor followed by analysis of cell invasion by transwell and scratch assays, LF15 protein expression and the relation between miR-512-5p and KLF15. miR-512-5p expression in exosomes was higher than glioma cells. Transfection of miR-512-5p derived from BMSCs decreased cell migration and invasion decreased the number of migrated and invaded cells. Combination of miR-512-5p and KLF15 inhibitor further decreased cell migration and invasion. Furthermore, miR-512-5p was confirmed to target KLF15 gene as show by a decreased luciferase activity. In conclusion, BMSCs secrete exosomes to encapsulate miR-512-5p, which inhibits KLF15 gene expression to exert an inhibitory effect on the invasion and migration of glioma cells.