Author:
Pang Long,Ding Jing,Liu Xi-Xian,Kou Zhixuan,Guo Lulu,Xu Xi,Fan Shih-Kang
Abstract
Intercellular interaction between cell–cell and cell–ECM is critical to numerous biology and medical studies, such as stem cell differentiation, immunotherapy and tissue engineering. Traditional methods employed for delving into intercellular interaction are limited by expensive equipment and sophisticated procedures. Microfluidics technique is considered as one of the powerful measures capable of precisely capturing and manipulating cells and achieving low reagent consumption and high throughput with decidedly integrated functional components. Over the past few years, microfluidics-based systems for intercellular interaction study at a single-cell level have become frequently adopted. This review focuses on microfluidic single-cell studies for intercellular interaction in a 2D or 3D environment with a variety of cell manipulating techniques and applications. The challenges to be overcome are highlighted.
Funder
Natural Science Foundation of Shaanxi Province
Natural Science Foundation of Shaanxi Provincial Department of Education
Subject
Cell Biology,Developmental Biology
Reference137 articles.
1. An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting.;Ahmadi;Lab Chip,2019
2. Single-cell metabolomics by mass spectrometry: advances, challenges, and future applications.;Ali;TrAC Trends Anal. Chem.,2019
3. Advances in microfluidics-based technologies for single cell culture.;Alonso;Adv. Biosyst.,2019
4. Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures.;Anagnostidis;Lab Chip,2020
5. Inverted open microwells for cell trapping, cell aggregate formation and parallel recovery of live cells.;Bocchi;Lab Chip,2012
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献