Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures-Reference-Cited by-同舟云学术

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

Published:2020 Issue:5 Volume:20 Page:889-900
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Anagnostidis Vasileios¹²³⁴,Sherlock Benjamin¹²³⁴,Metz Jeremy¹²³⁴,Mair Philip⁵⁶⁷⁴,Hollfelder Florian⁵⁶⁷⁴,Gielen Fabrice¹²³⁴^ORCID

Affiliation:

1. Living Systems Institute

2. University of Exeter

3. Exeter

4. UK

5. Department of Biochemistry

6. University of Cambridge

7. Cambridge

Abstract

To uncover the heterogeneity of cellular populations and multicellular constructs we show on-demand isolation of single mammalian cells and 3D cell cultures by coupling bright-field microdroplet imaging with real-time classification and sorting using convolutional neural networks.

Funder

Royal Society

Engineering and Physical Sciences Research Council

Wellcome Trust

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/D0LC00055H

Reference49 articles.

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