Alginate-Based 3D A549 Cell Culture Model to Study Paracoccidioides Infection
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Published:2023-05-31
Issue:6
Volume:9
Page:634
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
dos Santos Kelvin Sousa1ORCID, Oliveira Lariane Teodoro1, de Lima Fontes Marina1, Migliato Ketylin Fernanda2, Fusco-Almeida Ana Marisa1, Mendes Giannini Maria José Soares1ORCID, Moroz Andrei1
Affiliation:
1. Department of Clinical Analyses, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 85040-167, São Paulo, Brazil 2. Centro Universitário Central Paulista—UNICEP, São Carlos 13563-470, São Paulo, Brazil
Abstract
A three-dimensional (3D) lung aggregate model based on sodium alginate scaffolds was developed to study the interactions between Paracoccidioides brasiliensis (Pb) and lung epithelial cells. The suitability of the 3D aggregate as an infection model was examined using cell viability (cytotoxicity), metabolic activity, and proliferation assays. Several studies exemplify the similarity between 3D cell cultures and living organisms, which can generate complementary data due to the greater complexity observed in these designed models, compared to 2D cell cultures. A 3D cell culture system of human A549 lung cell line plus sodium alginate was used to create the scaffolds that were infected with Pb18. Our results showed low cytotoxicity, evidence of increased cell density (indicative of cell proliferation), and the maintenance of cell viability for seven days. The confocal analysis revealed viable yeast within the 3D scaffold, as demonstrated in the solid BHI Agar medium cultivation. Moreover, when ECM proteins were added to the alginate scaffolds, the number of retrieved fungi was significantly higher. Our results highlight that this 3D model may be promising for in vitro studies of host–pathogen interactions.
Funder
São Paulo Research Foundation CAPES
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference74 articles.
1. Organotypic 3D cell culture models: Using the rotating wall vessel to study host–pathogen interactions;Barrila;Nat. Rev. Microbiol.,2010 2. Barrila, J., Crabbé, A., Yang, J., Franco, K., Nydam, S.D., Forsyth, R.J., Davis, R.R., Gangaraju, S., Ott, C.M., and Coyne, C.B. (2018). Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age. Infect. Immun., 86. 3. Braz, J.D., Sardi, J.d.C.O., Pitangui, N.d.S., Voltan, A.R., Almeida, A.M.F., and Mendes-Giannini, M.J.S. (2021). Gene expression of Paracoccidioides virulence factors after interaction with macrophages and fibroblasts. Memórias Do Inst. Oswaldo Cruz, 116. 4. Merad, Y., Derrar, H., Belmokhtar, Z., and Belkacemi, M. (2021). Aspergillus Genus and Its Various Human Superficial and Cutaneous Features. Pathogens, 10. 5. Paracoccidioides-host Interaction: An Overview on Recent Advances in the Paracoccidioidomycosis;Assato;Front. Microbiol.,2015
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