Boswellia Essential Oil: Natural Antioxidant as an Effective Antimicrobial and Anti-Inflammatory Agent
-
Published:2023-09-27
Issue:10
Volume:12
Page:1807
-
ISSN:2076-3921
-
Container-title:Antioxidants
-
language:en
-
Short-container-title:Antioxidants
Author:
Obiștioiu Diana1ORCID, Hulea Anca2ORCID, Cocan Ileana3ORCID, Alexa Ersilia3, Negrea Monica3, Popescu Iuliana1ORCID, Herman Viorel2ORCID, Imbrea Ilinca Merima4, Heghedus-Mindru Gabriel3ORCID, Suleiman Mukhtar Adeiza5, Radulov Isidora1, Imbrea Florin1
Affiliation:
1. Faculty of Agriculture, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania 2. Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania 3. Faculty of Food Engineering, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania 4. Faculty of Engineering and Applied Technologies, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania 5. Faculty of Life Science, Department of Biochemistry, Ahmadu Bello University, Zaria 810107, Kaduna State, Nigeria
Abstract
The research aimed to determine the chemical composition, the antioxidant and anti-inflammatory activity as well as the antimicrobial activity against Gram-positive, Gram-negative and two fungal Candida ATCC strains of a commercial Boswellia essential oil (BEO) containing Boswellia carteri, Boswellia sacra, Boswellia papryfera, and Boswellia frereana. Additionally, molecular docking was carried out to show the molecular dynamics of the compounds identified from the essential oil against three bacterial protein targets and one fungal protein target. The major components identified by GC-MS (Gas Chromatography-Mass Spectrometry) were represented by α-pinene, followed by limonene. Evaluation of antioxidant activity using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method showed high inhibition comparable to the synthetic antioxidant used as a control. Oxidative stability evaluation showed that BEO has the potential to inhibit primary and secondary oxidation products with almost the same efficacy as butylated hydroxyanisole (BHA). The use of BEO at a concentration of 500 ppm provided the best protection against secondary oxidation during 30 days of storage at room temperature, which was also evident in the peroxide value. Regarding the in vitro anti-inflammatory activity, the membrane lysis assay and the protein denaturation test revealed that even if the value of protection was lower than the value registered in the case of dexamethasone, the recommendation of using BEO as a protective agent stands, considering the lower side effects. Gram-positive bacteria proved more sensitive, while Pseudomonas aeruginosa presented different sensitivity, with higher MICs (minimal inhibitory concentration). Haemophilus influenzae demonstrated a MIC at 2% but with consecutive inhibitory values in a negative correlation with the increase in concentration, in contrast to E. coli, which demonstrated low inhibitory rates at high concentrations of BEO. The computational tools employed revealed interesting binding energies with compounds having low abundance. The interaction of these compounds and the proteins (tyrosyl-tRNA synthetase, DNA gyrase, peptide deformylase, 1,3-β-glucan synthase) predicts hydrogen bonds with amino acid residues, which are reported in the active sites of the proteins. Even so, compounds with low abundance in BEO could render the desired bioactive properties to the overall function of the oil sustained by physical factors such as storage and temperature. Interestingly, the findings from this study demonstrated the antioxidant and antimicrobial potential of Boswellia essential oil against food-related pathogens, thus making the oil a good candidate for usage in food, feed or food-safety-related products.
Funder
Increasing the impact of excellence research on the capacity for innovation and technology transfer within USAMVB Timisoara
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference83 articles.
1. Tetra- and Pentacyclic Triterpene Acids from the Ancient Anti-Inflammatory Remedy Frankincense as Inhibitors of Microsomal Prostaglandin E 2 Synthase-1;Verhoff;J. Nat. Prod.,2014 2. Phytochemistry and Potential Therapeutic Actions of Boswellic Acids: A Mini-Review;Iram;Asian Pac. J. Trop. Biomed.,2017 3. Chemical Composition, Antibacterial Activity, and Antibiotic Potentiation of Boswellia Sacra Flueck. Oleoresin Extracts from the Dhofar Region of Oman;Rashan;Evid.-Based Complement. Altern. Med.,2021 4. Effect of Boswellia Species on the Metabolic Syndrome: A Review;Mahdian;Iran. J. Basic Med. Sci.,2020 5. Di Stefano, V., Schillaci, D., Cusimano, M.G., Rishan, M., and Rashan, L. (2020). In Vitro Antimicrobial Activity of Frankincense Oils from Boswellia Sacra Grown in Different Locations of the Dhofar Region (Oman). Antibiotics, 9.
|
|