Nitric Oxide‐Releasing Poly(L‐glutamic acid) Hybrid Hydrogels for Accelerating Diabetic Wound Healing†

Abstract

Comprehensive SummaryDiabetic wound healing is threatening worldwide and challenging in wound dressings. Aiming to inhibit heavy inflammation and bacterial infection in diabetic wounds, herein, we facilely construct a kind of NO‐releasing poly(L‐glutamic acid) (PGA) based graphene oxide (GO) hybrid hydrogel of HDGS at a lower solid percentage, presenting large microporous size of about 20 μm, mild photothermal conversion property, and good biocompatibility. Besides improving hemostasis performance, the less amount of GO endowed the hydrogel with near infrared (NIR) responsivity to control fast and pulsatile NO release for killing bacteria and inhibiting heavy inflammation to proheal diabetic wound, in which a broad spectrum of antibacterial activities toward killing S. aureus, E. coli and MRSA was achieved via a combined effect of photothermia and NO release. In vivo effective hemostasis was attained in a rat liver bleeding model with short hemostatic time of ~20 s and lower blood loss of 1.5%—2.0%. Moreover, the treatment of HDGS plus 4 times of mild NIR irradiation (10 min, 808 nm, 1 W/cm2 per time) performed superior full diabetic wound healing within 11—14 d, in which the regenerated skins were characteristic of thick epidermis/dermis, dense blood vessels, some hair follicles‐embedding, and high level of collagens.