Optimized Hemolysin Type 1 Secretion System in <i>Escherichia coli</i> by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region-Reference-Cited by-同舟云学术

Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region

Published:2022-02-08 Issue:6 Volume:23 Page:
ISSN:1439-4227
Container-title:ChemBioChem
language:en
Short-container-title:ChemBioChem

Author:

Pourhassan N. Zohreh¹,Cui Haiyang²³⁴^ORCID,Khosa Sakshi¹,Davari Mehdi D.⁵,Jaeger Karl‐Erich⁶,Smits Sander H. J.¹,Schwaneberg Ulrich²³,Schmitt Lutz¹^ORCID

Affiliation:

1. Institute of Biochemistry Heinrich Heine University Universitätsstrasse 1 40225 Düsseldorf Germany

2. Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

3. DWI-Leibniz Institute for Interactive Materials Forckenbeckstraße 50 52056 Aachen Germany

4. Present address: Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana-Champaign 1206 West Gregory Drive Urbana Il 61801 USA

5. Department of Bioorganic Chemistry Leibniz Institute of Plant Biochemistry Weinberg 3 06120 Halle Germany

6. Institute of Molecular Enzyme Technology Heinrich Heine University and Institute of Bio- and Geosciences IBG-1 Biotechnology, Forschungszentrum Jülich GmbH 52426 Jülich Germany

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Organic Chemistry,Molecular Biology,Molecular Medicine,Biochemistry

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.202100702

Reference55 articles.

1. Understanding and Managing the Biotechnology Valley of Death

2. Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

3. Escherichia coli as an Experimental Organism

4. Recombinant protein expression in Escherichia coli: advances and challenges

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1. A step forward to the optimized HlyA type 1 secretion system through directed evolution;Applied Microbiology and Biotechnology;2023-07-05

2. Investigations on the substrate binding sites of hemolysin B, an ABC transporter, of a type 1 secretion system;Frontiers in Microbiology;2022-12-01

3. Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region;ChemBioChem;2022-02-08