Affiliation:
1. College of Food Science and Technology Nanjing Agricultural University Nanjing China
2. College of Tea and Food Science Technology Jiangsu Vocational College of Agriculture and Forestry Jurong China
Abstract
AbstractOxidative stress is a key process in the development of neurodegenerative diseases. More attention is needed to screen natural antioxidants and explore pharmacological mechanisms. Natural product polysaccharides with no toxic side effects have powerful antioxidant activity. Two purified intracellular polysaccharide fractions (IPS1 and IPS2) from Paecilomyces cicadae TJJ1213 was isolated. Then, a model of H2O2‐induced oxidative stress in PC12 cells was established to investigate the neuroprotective role of IPS and elucidate the potential protection mechanism. Results showed that IPS1 and IPS2 reduced reactive oxygen species (ROS) production, inhibited the leakage of lactate dehydrogenase (LDH) and Ca2+ and attenuated the expression of apoptotic proteins. In addition, western blots displayed that IPS1 and IPS2 significantly inhibited mitophagy induced by H2O2 in PC12 cells via PINK/Parkin pathway. Therefore, IPS1 and IPS2 deserved further investigation as protective agents against neurodegenerative diseases.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Jiangsu Province
Subject
Molecular Medicine,Biochemistry,Drug Discovery,Pharmacology,Organic Chemistry
Cited by
1 articles.
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