Affiliation:
1. Department of Biochemistry, School of Biosciences Periyar University Salem India
2. Dhanvanthri Laboratory, Department of Sciences Amrita School of Physical Science Amrita Vishwa Vidyapeetham Coimbatore 641112 India
3. Center of Excellence in Advanced Materials & Green Technologies (CoE–AMGT) Amrita School of Engineering Amrita Vishwa Vidyapeetham Coimbatore 641112 India
4. Centro Tecnológico de la Carne de Galicia Avd. Galicia No. 4, Parque Tecnológico de Galicia, San Cibrao das Viñas 32900 Ourense Spain
5. Área de Tecnoloxía dos Alimentos, Facultade de Ciencias Universidade de Vigo 32004 Ourense Spain
Abstract
SummaryA polysaccharide (HUP1) was extracted and purified from Hypsizygus ulmarius fruiting bodies and further acetylated (Ac‐HUP1) to improve their physicochemical properties and bioactivities. Chemical and FT‐IR spectrum analyses results indicated differences in functionality and activity between HUP1 and Ac‐HUP1, demonstrating the efficacy of the acetylation. HUP1 has an acetyl group with a degree of substitution (DS) 0.34. The molecular weight of the modified acetylated derivatives was reduced, according to the HPSEC findings. The native and acetylated polysaccharides included varying molar percentages of Ara, Man, Glu, GlcA, and GalA. The Ac‐HUP1 outperformed HUP1 in antioxidant activity using the radical scavengers ABTS, DMPD, DPPH, superoxide, and OH radicals. In addition, Ac‐HUP1 demonstrated higher activity in reducing power using Prussian blue, cupric ion, ferric ion, and phosphomolybdenum, as well as in lipid peroxidation inhibition assay. HUP1 and Ac‐HUP1 inhibited five cancer cells (HT29, PC3, HeLa, Jurkat, and HepG‐2) at concentrations from 10 to 320 μg mL−1, and the impact was more pronounced in acetylated derivative, which was determined by MTT assay. Ac‐HUP1, compared to HUP1, considerably prolonged the APTT, PT, and TT exhibiting anticoagulant activities. Therefore, acetylation modification may improve polysaccharide'sphysicochemical and biological characteristics, allowing them to be employed in the food, nutraceutical, and pharmaceutical industries.
Subject
Industrial and Manufacturing Engineering,Food Science
Cited by
4 articles.
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