Improving properties of platelet‐rich fibrin scaffold with tannic acid for wound healing

Author:

Haghparast‐Kenarsari Tahereh1,Abdouss Majid2,Heidari Keshel Saeed3,Heshmatipour Zoheir4,Rahimi Azam3,Biazar Esmaeil5ORCID

Affiliation:

1. Department of Biomedical Engineering, Central Tehran Branch Islamic Azad University Tehran Iran

2. Department of Chemistry Amirkabir University of Technology (AUT) Tehran Iran

3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran

4. Department of Microbiology, Tonekabon Branch Islamic Azad University Tonekabon Iran

5. Tissue Engineering Group, Department of Biomedical Engineering, Tonekabon Branch Islamic Azad University Tonekabon Iran

Abstract

AbstractPlatelet‐rich fibrin (PRF), which is the rich source of growth factors, has been used as an efficient scaffold in tissue engineering and wound healing. In this study, tannic acid as a green cross‐linker with different concentrations (0.5%, 1%, 5% and 10%) was used to improve the properties of PRF. The cross‐linked gel scaffolds were evaluated by analyses such as scanning electron microscopy, Fourier transform infrared spectroscopy, swelling and degradation, mechanical strength, cell toxicity, cell adhesion and antibacterial test. The results showed that the scaffold structure changes by increasing cross‐linker concentration. The swelling rate decreased from 49% to 5% for the samples without the cross‐linker and with tannic acid (10%), respectively. The degradation percentage for the cross‐linked samples was 8%, which showed a lower degradation rate than the non‐cross‐linked samples (63%). The mechanical strength of the scaffold with the cross‐linker increased up to three times (Young's modulus for the non‐cross linked and the cross‐linked samples: 0.01 and 0.6 MPa, respectively). Cytotoxicity was not observed up to 10% cross‐linker concentration. The cells proliferated well on the cross‐linked scaffolds and also showed a good antibacterial effect. In general, tannic acid can improve the physical and mechanical properties of PRF without negatively affecting its biological properties.

Publisher

Wiley

Subject

Dermatology,Surgery

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3