Affiliation:
1. Department of Dermatology, Southwest Hospital Army Medical University Chongqing China
2. Institute of Immunology PLA & Department of Immunology Army Medical University Chongqing China
3. Department of Immunology, College of Basic Medicine Qingdao University Qingdao China
4. Department of Immunology, College of Basic Medicine Weifang Medical University Weifang China
Abstract
AbstractBackgroundAtopic dermatitis (AD) is a chronic and recurrent inflammatory skin disease characterized by severe pruritus and eczematous lesions. Heterogeneity of AD has been reported among different racial groups according to clinical, molecular and genetic differences.ObjectiveThis study aimed to conduct an in‐depth transcriptome analysis of AD in Chinese population.MethodsWe performed single‐cell RNA sequencing (scRNA‐seq) analysis of skin biopsies from five Chinese adult patients with chronic AD and from four healthy controls, combined with multiplexed immunohistochemical analysis in whole‐tissue skin biopsies. We explored the functions of IL19 in vitro.ResultsScRNA‐seq analysis was able to profile a total of 87,853 cells, with keratinocytes (KCs) in AD manifesting highly expressed keratinocyte activation and pro‐inflammatory genes. KCs demonstrated a novel IL19+IGFL1+ subpopulation that increased in AD lesions. Inflammatory cytokines IFNG, IL13, IL26 and IL22 were highly expressed in AD lesions. In vitro, IL19 directly downregulated KRT10 and LOR in HaCaT cells and activated HaCaT cells to produce TSLP.ConclusionAbnormal proliferation and differentiation of keratinocytes contribute immensely to the pathogenesis of AD, whereas AD chronic lesions have witnessed significant presence of IL19+IGFL1+KCs, which may be involved in the disruption of the skin barrier, the connection and magnification of Th2 and Th17 inflammatory responses, and mediation of skin pruritus. Furthermore, progressive activation of multiple immune axes dominated by Type 2 inflammatory reaction occur in AD chronic lesions.
Funder
National Natural Science Foundation of China
Subject
Infectious Diseases,Dermatology