Affiliation:
1. Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland
2. Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA
Abstract
Six
Staphylococcus
strains were isolated from healthy black bears (Ursus americanus) in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, dnaJ, hsp60, rpoB and sodA genes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the ‘
Staphylococcus intermedius
group’ (SIG), which include
Staphylococcus intermedius
, Staphylococcus pseudintermedius, Staphylococcus delphini and Staphyloccoccus cornubiensis. The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the nuc gene. The strains can be differentiated from the other SIG species by the absence of sucrose fermentation, from
S. intermedius
DSM 20373T,
S. pseudintermedius
CCUG 49543T and
S. cornubiensis
DSM 105366T by the absence of methyl β-d-glucopyranoside fermentation and from
S. delphini
DSM 20771T by fermentation of trehalose. DNA relatedness of the type strain MI 10-1553T with the type strains of
S. delphini
,
S. pseudintermedius
,
S. intermedius
and
S. cornubiensis
was ≤48.2 % by digital DNA–DNA hybridization and ≤92.3 % by average nucleotide identity calculations. Iso-C15:0, anteiso-C15 : 0 and anteiso-C17 : 0 were the most common fatty acids. Polar lipids consisted of phosphadidylglycerols, phospholipids, glycolipid, diphosphatidylglycerol and aminophospholipid. Cell-wall peptidoglycan was of type A3α l-Lys-Gly3 (Ser; similar to A11.2 and A11.3). The respiratory quinone belonged to menaquinone 7 (MK-7). The G+C content of MI 10-1553T was 39.3 mol%. The isolated strains represent a novel species of the genus
Staphylococcus
, for which we propose the name Staphylococcus ursi sp. nov. The type strain is MI 10-1553T (=ATCC TSD-55T=CCOS 1900T).
Funder
All Taxa Biodiversity Inventory Grant from Discover Life in America
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
18 articles.
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