Affiliation:
1. Chinese Academy of Sciences
2. Shandong University
3. Xuzhou Medical University
4. Beijing Drainage Group Co., Ltd
Abstract
Abstract
A strictly anaerobic, motile bacterium, designated as strain Ai-910T, was isolated from the sludge of an anaerobic digestion tank in China. Cells were Gram-stain-negative rods. Optimal growth was observed at 38°C (growth range 25–42°C), pH 8.5 (growth range 5.5–10.5), and under a NaCl concentration of 0.06% (w/v) (range 0–2.0%). Major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The respiratory quinone was MK-7. Using xylose as the growth substrate, succinate was produced as the fermentation product. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain Ai-910T formed a distinct phylogenetic lineage that reflects a new genus in the family Marinilabiliaceae, sharing high similarities to Alkaliflexus imshenetskii Z-7010T (92.78%), Alkalitalea saponilacus SC/BZ-SP2T (92.51%), and Geofilum rubicundum JAM-BA0501T (92.36%). Genomic similarity (average nucleotide identity and digital DNA-DNA hybridization) values between strain Ai-910T and its phylogenetic neighbors were below 65.27 and 16.90%, respectively, indicating that strain Ai-910T represented a novel species. The average amino acid identity between strain Ai-910T and other related members of the family Marinilabiliaceae were below 69.41%, supporting that strain Ai-910T was a member of a new genus within the family Marinilabiliaceae. Phylogenetic, genomic, and phenotypic analysis revealed that strain Ai-910T was distinguished from other phylogenetic relatives within the family Marinilabiliaceae. The genome size was 3.10 Mbp, and the DNA G + C content of the isolate was 42.8 mol%. Collectively, differences of the phenotypic and phylogenetic features of strain Ai-910T from its close relatives suggest that strain Ai-910T represented a novel species in a new genus of the family Marinilabiliaceae, for which the name Xiashengella succiniciproducens gen. nov., sp. nov. was proposed. The type strain of Xiashengella succiniciproducens is Ai-910T (= CGMCC 1.17893T = KCTC 25304T).
Publisher
Research Square Platform LLC
Reference52 articles.
1. DSMZ medium 287. https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium287.pdf. Accessed 4 May 2016.
2. Pararoseburia lenta gen. nov., sp. nov. isolated from human faeces;Abdugheni R;Int J Syst Evol Microbiol,2022
3. Abdugheni R, Li DH, Wang YJ, Du MX, Zhou N, Liu C, et al. (2023) Acidaminococcus hominis sp. nov., Amedibacillus hominis sp. nov., Lientehia hominis gen. nov. sp. nov., Merdimmobilis hominis gen. nov. sp. nov., and Paraeggerthella hominis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 73 doi: 10.1099/ijsem.0.005648
4. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov;Bowman JP;Int J Syst Evol Microbiol 50 Pt,2000
5. BPGA- an ultra-fast pan-genome analysis pipeline;Chaudhari NM;Sci Rep,2016