The expression and regulation of the nuclear receptor-binding SET domain protein 2 in parotid carcinoma

Abstract

Abstract Background To explore the effect of down-regulating NSD2 gene expression on proliferation, apoptosis and invasion of parotid carcinoma. Materials and methods SACC-2 cells were transfected with NSD2 siRNA. Real-time quantitative PCR was used to detect expression of NSD2 mRNA and western blot was used to detect the expression of NSD2 protein. CCK-8 and colony formation test were used to detect cell proliferation. Annexin V PI double staining flow cytometry was used to detect apoptosis, cell scratch and transwell test were used to detect cell invasion. Results The apoptosis rates of siNSD2-1 and siNSD2-2 groups were significantly higher. The wounds of siNSD2-1 and siNSD2-2 group were more significant than that of control, and relative migration distances were reduced significantly than that of control (P < 0.05). The cells which penetrated cell membrane of siNSD2-1 and siNSD2-2 were significantly less than those of control (P < 0.05). Conclusions Silencing NSD2 gene can inhibit the proliferation and invasion of parotid carcinoma SACC-2 cells and induce apoptosis.