Upregulation of Twist is involved in Gli1 induced migration and invasion of hepatocarcinoma cells

Author:

Li Juan1,He Yuting1,Cao Yuan2,Yu Yan1,Chen Xiaolong1,Gao Xiaojuan1,Hu Qiuyue1

Affiliation:

1. Department of Infectious Diseases , The First Affiliated Hospital of Zhengzhou University , Zhengzhou 450052 , China

2. Department of Gynecology and Obstetrics , The First Affiliated Hospital of Zhengzhou University , Zhengzhou 450052 , China

Abstract

Abstract Hedgehog (Hh) signaling is involved in the progression of hepatocellular carcinoma (HCC), while its detailed mechanisms are not well illustrated. Our present study revealed that the expression of Gli1, while not Gli2 or Gli3, is significantly increased in HCC cell lines and 20/28 (71.4%) HCC tissues as compared with their corresponding controls. Over expression of Gli1 can promote the migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells. Gli1 can increase the expression of Twist, while not other EMT transcription factors such as Snail, ZEB1 or Slug. Gli1 increases the transcription of Twist while it has no significant effect on the protein or mRNA stability. Chromatin immunoprecipitation-polymerase chain reaction confirms that Gli1 can directly bind to the promoter of Twist, in which the third binding site is essential for Gli1 induced transcription. Collectively, our data suggest that upregulation of Twist is involved in Gli1 induced migration and invasion of HCC cells.

Funder

National Natural Science Foundation of China

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

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