Affiliation:
1. Faculty of Environmental and Forest Biology (K.D.C., L.B.S.) and Faculty of Chemistry (M.A.T.), State University of New York, College of Environmental Science and Forestry, Syracuse, New York 13210
Abstract
Abstract
Cuticular wax deposition and composition affects drought tolerance and yield in plants. We examined the relationship between wax and dehydration stress by characterizing the leaf cuticular wax of tree tobacco (Nicotiana glauca L. Graham) grown under periodic dehydration stress. Total leaf cuticular wax load increased after each of three periods of dehydration stress using a CH2Cl2 extraction process. Overall, total wax load increased 1.5- to 2.5-fold, but composition of the wax was not altered. Homologous series of wax components were classified into organic groups; n-hentriacontane was the largest component (>75%) with alcohols and fatty acids representing <10% of the entire wax load. An increase in density, but no change in the three-dimensional shape, of leaf wax crystals was evident under low-kV scanning electron microscopy after each drying event. Leaves excised from plants subjected to multiple drying events were more resistant to water loss compared to leaves excised from well-watered plants, indicating that there is a negative relationship between total wax load and epidermal conductance. Lipid transfer proteins (LTPs) are thought to be involved in the transfer of lipids through the extracellular matrix for the formation of cuticular wax. Using northern analysis, a 6-fold increase of tree tobacco LTP gene transcripts was observed after three drying events, providing further evidence that LTP is involved in cuticle deposition. The simplicity of wax composition and the dramatic wax bloom displayed by tree tobacco make this an excellent species in which to study the relationship between leaf wax deposition and drought tolerance.
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Reference74 articles.
1. Baker E (1982) Chemistry and morphology of plant epicuticular waxes. In D Cutler, K Alvin, C Price, eds, The Plant Cuticle. Academic Press, New York, pp 139–165
2. Baur P, Marzouk H, Schönherr J (1999) Estimation of path lengths for diffusion of organic compounds through leaf cuticles. Plant Cell Environ22:291–299
3. Becker M, Kerstiens G, Schönherr J (1986) Water permeability of plant cuticles: permeance, diffusion and partition coefficients. Trees1:54–60
4. Bengtson C, Larsson S, Liljenberg C (1978) Effects of water stress on cuticular transpiration rate and amount and composition of epicuticular wax in seedlings of six oat varieties. Physiol Plant44:319–324
5. Bianchi G (1995) Plant waxes. In RJ Hamilton, ed, Waxes: Chemistry, Molecular Biology and Functions. Oily Press, Dundee, Scotland, pp 176–222
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