Detection of protein content in alfalfa using visible/ near-infrared spectroscopy technology

Author:

Li Jie1,Wu Guifang1,Guo Fang1,Han Lei1,Xiao Haowen2,Cao Yang2,Yang Huihe1,Yan Shubin1

Affiliation:

1. Inner Mongolia Agricultural University

2. Inner Mongolia Autonomous Region Agricultural and Pastoral Technology Extension Center

Abstract

In this study, a quantitative model was developed using near-infrared spectroscopy to analyze protein content in dried purple alfalfa, employing preprocessing methods (SG, SNV, MSC, FD) and variable selection algorithms (CARS, IRIV) to optimize spectra. Models using ELM, PLSR, SVM, and LSTM were tested; the MSC-CARS-PLSR-SVM model achieved the highest accuracy, with a calibration determination coefficient (R²) of 0.9982 and root mean square error (RMSE) of 0.1088, and a prediction R² of 0.9645 with RMSE of 0.5230, offering a precise and reliable method for protein content prediction.

Publisher

BioResources

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