Central Composite Design: a Response Surface Methodology Approach in Biodegradation of Textile Dye Wastewater

Abstract

Objectives : This study evaluated and identified the removal of colors and chemocal oxygen demands from thextile dye effluents by Bacillus cereus isolated from the local textile wastewater treatment plant.Methods : Central composite design (CCD) from response surface methodology (RSM) was applied in order to achieve the optimized treatment process condition for the textile dyes wastewater degradation. Two-level of three process parameters with six center points resulted a total of twenty runs of experiments were performed. Bacterial inoculum (-1,+1) (%, v/v), agitation (-1, +1) (rpm), and pH (-1, +1) were tested.Results and Discussion : During the ten days of biodegradation process, highest decolourization achieved was 88.67% with low pH and agitation; and medium level of initial concentration of bacterial inoculum. Highest chemical oxygen demand (COD) removal was achieved with 99.20% from high pH (pH 10), low agitation (100 rpm) and high initial concentration of bacterial inoculum (15%, v/v).Conclusions : The biological treatments was able to remove colour and chemical oxygen demand with application of CCD, giving the optimum settings of the three process parameters studied.