Force Vectors of Single Motor Units in a Multipennate Muscle

Abstract

The masseter muscle of the rabbit has a complex architectural design. Restricted motor unit territories in the muscle provide an anatomic basis for accurate control of the force vector through selective activation. In addition, the muscle shows regional differences in fiber type composition. The main objective of the present study was to measure the force vectors of single motor units within the rabbit masseter muscle by a direct mechanical approach to test the hypothesis that: (1) motor units within the masseter muscle are capable of generating different force vectors; and (2) different motor unit types are distributed heterogeneously throughout the muscle. We used a force transducer, capable of measuring both the magnitude and the position of the line of action of a force in a single plane. Motor units in the masseter muscle showed a large range of twitch contraction times and force magnitudes. There was also a large variation in the direction and moment arm of the lines of action. The variation of the lines of action was (almost) as large as the range of fiber directions found inside the muscle. Largest forces, with relatively slow contraction velocities, were produced by motor units in the anterior masseter. Smaller forces and fastest twitch contractions were produced by motor units in the posterior deep masseter. In addition, motor units in the anterior masseter showed more variability in force production than in the posterior masseter. Our results support the idea that the masseter muscle is divided into functionally different parts.