Affiliation:
1. Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
2. Institute of Materia Medicines, Zhejiang Academy of Medical Sciences, Hangzhou, China.
3. Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou, China.
Abstract
Salidroside (SDS) has been reported to have anti-inflammatory properties. The objective of this study was to investigate the protective effect of SDS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. BALB/c mice were pretreated with SDS 1 hour before intranasal instillation of LPS. Seven hours after LPS administration, the myeloperoxidase in histology of lungs, lung wet/dry ratio, and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of pro-inflammatory cytokines, tumor necrosis factor α (TNF-α), interleukin-1β (IL 1β), and IL-6 in the BALF were measured by enzyme-linked immunosorbent assay. The expression of Toll-like receptor 4 (TLR4), inhibitor of nuclear factor-kappa B (IκB-α), and nuclear factor-kappa B (NF-κB) p65 was detected by Western blot. The SDS reduced the inflammatory cells in BALF, decreased the wet/dry ratio of lungs, attenuated the LPS-induced histological alterations in the lung, and inhibited the production of TNF-α, IL-1β, and IL-6. Western blot showed that SDS efficiently inhibited the phosphorylation of IκB-α, p65 NF-κB, and the expression of TLR4. These data show that the anti-inflammatory effects of SDS (at least 20 mg/kg) against LPS-induced ALI due to its ability to inhibit TLR4 mediated the NF-κB signaling pathways. The SDS may represent a novel strategy for treating LPS-induced ALI.
Subject
Chemical Health and Safety,Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Toxicology
Cited by
17 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献