Influence of Cellulases on Indigo Backstaining

Author:

Andreaus Jürgen1,Campos Rui1,Gübitz Georg2,Cavaco-Paulo Artur3

Affiliation:

1. Textil Alberto de Sousa, S.A., V. N. de Sande, 4802-Guimarães, Portugal

2. Technical University of Graz, Graz. Austria

3. Departamento de Engenharia Textil, Universidade do Minho, 4800-Guimarães, Portugal

Abstract

We have found that increasing concentrations of fungal cellulases on a fabric decrease indigo staining levels. Deletion of the cellulose binding domains (CBD) from either bacterial or fungal cellulases decreases indigo staining levels and generally causes less backstaining than the entire enzyme. Increasing the concentration of cellulases with a CBD of family I on fabric decreases indigo staining, whereas increasing the concentration of cellulase with a CBD of family II has no effect on staining. After-washing experiments of indigo-stained cotton fabrics show that it is easier to remove indigo adsorbed on cellulase adsorbed onto cotton than indigo directly adsorbed onto cotton.

Publisher

SAGE Publications

Subject

Polymers and Plastics,Chemical Engineering (miscellaneous)

Reference9 articles.

1. Boraston, A., Bray, M., Brun, E., Creagh, A.L., Gilkes, N.R., Guarna, M.M., Jervis, E., Johnson, P., Kormos, J., McIntosh, L., McLean, B.W., Sandercocck, L.E., Tomme, P., Haynes, C.A., Warren, R.A.J., and Kilburn, D.G., The Structure and Function of Cellulose Binding Domains, in "Carbohydratases from Trichoderma reesei and Other Microorganisms," M. Claeyssens, W. Nerinckx, and K. Piens, Eds. The Royal Society of Chemistry , Cambridge, 1998, pp. 139-146.

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

3. Indigo-Cellulase Interactions

4. Interactions of cotton with CBD peptides

5. Indigo Backstaining During Cellulase Washing

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