The Lineages of Neuroglial Cells

Abstract

The study of neuroglial cell lineages in the CNS identifies the time in development, when astrocytes and oligodendrocytes diverge from a common precursor cell. Recent studies using retroviral tracing show that the lineages for astrocytes and oligodendrocytes begin to diverge as early as embryonic day 13 (E13) in the cerebellum and as early as E15 in the forebrain. A very small percentage of glial precursor cells present in late pre- and postnatal development are pluripotential, but the vast majority of astrocytes and oligodendrocytes in the brain are derived from “committed” precursors. The precursors for these postmitotic astrocytes and oligodendrocytes are immature astrocytes and oligodendrocytes (progenitors) that express molecular properties unique to each of these cell types. It is critical to distinguish glial lineage (thought of in terms of a glial cell's ancestry in normal development) from glial plasticity (the potential of a glial cell to alter its fate when its normal environment is changed). In tissue culture, a bipotential cell known as the O-2A cell generates oligodendrocytes under one set of culture conditions and retains plasticity to become a subtype of astrocyte under another set. Whether all cells phenotyped as O-2A cells in culture are bipotential or whether only a subset displays this capacity is still unclear.