Effect of Probiotic Fermentation on Antinutrients and In Vitro Protein and Starch Digestibilities of Indigenously Developed RWGT Food Mixture

Abstract

Indigenously developed RWGT food mixture which contained rice flour, whey, sprouted green gram paste and tomato pulp (2∶1∶1∶1 w/w) was autoclaved 1.5 kg/cm2, 15 min, 121°C), cooled and fermented with 2% liquid culture (containing 106 cells/ml broth). Two types of fermentations were carried out i.e. single culture fermentation [ L. casei, L. plantarum (37°C, 24 hr.)] and sequential culture fermentation [ S. boulardii (25°C, 24 hr.) + L. casei (37°C, 24 hr.)]; S. boulardii [(25°C, 24 hr.) + L. plantarum (37°C, 24 hr.)]. All the fermentations drastically reduced the contents of phytic acid, polyphenols and trypsin inhibitor activity while significantly improving the in vitro digestibilities of starch and protein. Sequential culture fermentations brought about higher changes as compared to single culture fermentations.