Intrabeam Radiation Inhibits Proliferation, Migration, and Invasiveness and Promotes Apoptosis of MCF-7 Breast Cancer Cells

Author:

Pan Lingxiao12,Wan Minghui3,Zheng Wenbo2,Wu Rui4,Tang Wei2,Zhang Xiaoshen2,Yang Tong5,Ye Changsheng1

Affiliation:

1. Breast Center, Nanfang Hospital, Southern Medical University, Guangzhou, China

2. Department of Breast Surgery, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

3. Department of Radiation Oncology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

4. Department of Radiotherapy, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

5. Department of Pathology, the Second Affiliated Hospital (Panyu branch) of Guangzhou Medical University, Guangzhou, China

Abstract

Intraoperative radiotherapy differs from the more commonly used external beam radiation with respect to fractionation, radiation energy, dose rate, and target volume, which may influence the irradiated cells in a complex manner. However, experimental studies of intraoperative radiotherapy are limited. Intrabeam is a frequently used intraoperative radiotherapy device; we evaluated its effects on the proliferation, apoptosis, migration, and invasion of MCF-7 human breast cancer cells. We performed colony formation assays for cells irradiated with single radiation doses of 0 to 16 Gy. Other cells were irradiated with single radiation doses of 0 to 6 Gy and then continued to be cultured. We measured cell-cycle distributions and apoptosis rates 24 hours later, using flow cytometry, and performed wound-healing assays, Transwell tests, and terminal deoxynucleotidyl transferase–mediated 2′-deoxyuridine 5′-triphosphate nick-end labeling staining 4 weeks later. Colony formation assays showed no positive colonies from cells irradiated with doses of ≥6 Gy. In flow cytometry, the experimental groups had higher late-apoptosis/necrosis rates ( P < .01) and higher percentages of cells arrested in G1 phase ( P < .01). Experimental groups also had much lower scratch-repair rates in the wound healing assay ( P < .001) and higher apoptosis rates in the terminal deoxynucleotidyl transferase–mediated 2′-deoxyuridine 5′-triphosphate nick-end labeling assay ( P < .05). In Transwell tests, the 4 Gy and 6 Gy groups had fewer invading cells than the control group ( P < .05). Single-dose irradiation of 6 Gy with the Intrabeam device can effectively inhibit proliferation, migration, and invasiveness and promote apoptosis in MCF-7 cells with long-lasting effects.

Funder

the Health and Family Planning Technology Project of Guangzhou Municipal Health and Family Planning Bureau

Publisher

SAGE Publications

Subject

Cancer Research,Oncology

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