Author:
Abdelkafy Wael M.,Smith Johnathon Q.,Henriquez Oswaldo A.,Golub Justin S.,Xu Jianguo,Rojas Mauricio,Brigham Kenneth L.,Johns Michael M.
Abstract
Objectives: Changes in voice are commonly associated with aging (presbyphonia). Age-related voice change significantly impairs elderly individuals' ability to communicate meaningfully with others and affects their quality of life. With changing age demographics in our society and increasing emphasis on quality of life, treatment of presbylaryngis is becoming more paramount. Methods: We used 9 aged and 9 young mice to validate a mouse model for the aging larynx. We stained the larynges with Alcian blue to determine the hyaluronic acid content, trichrome stain to determine the collagen content, and immunohis-tochemical stain for α smooth muscle actin to determine the myofibroblast content. Morphometric measurements were performed for muscle area, muscle thickness, and muscle fiber diameter. Results: Statistically significant differences in the density measurements of hyaluronic acid and collagen reflected decreased hyaluronic acid and increased collagen content in the aging larynx. We found α smooth muscle actin–labeled myofibroblasts only in the aged larynges. No statistically significant differences were found in the morphometric measurements. Conclusions: Aged mice may make a practical model for the age-related changes in the vocal folds that can be used further in studies aiming to correct these changes.
Subject
General Medicine,Otorhinolaryngology
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献