Proliferation of endothelial cells (HUVEC) on specific-modified collagen sponges loaded with different growth factors

Author:

Groger Andreas1,Megas Ioannis-Fivos2,Noah Ernst Magnus13,Pallua Norbert1,Grieb Gerrit12ORCID

Affiliation:

1. Department of Plastic Surgery and Hand Surgery, Burn Center, Medical Faculty, RWTH Aachen University, Aachen, Germany

2. Department of Plastic Surgery and Hand Surgery, Gemeinschaftskrankenhaus Havelhöhe, Berlin, Germany

3. Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, Rotes Kreuz Krankenhaus Kassel, Kassel, Germany

Abstract

In general, matrices for tissue engineering must maintain structural integrity during the process of tissue formation and promote vascularization of developing tissue. Therefore, collagen sponges, manufactured by an approach that offers the potential of unidirectional pore size, were seeded with human umbilical vein endothelial cells (HUVEC) to demonstrate a positive effect on cell proliferation. In addition, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) have been used to promote proliferation of HUVEC on optimized collagen sponges. Growth and viability of the cells were evaluated. Potential unidirectional pore structure demonstrated an improvement of both, endothelial cell growth and viability. Supplementation of growth factors showed an additional increase of endothelial cell growth on collagen sponges, which confirmed the high potential of combining this biomaterial with growth factors. The results suggest that a collagen sponge with a potential specific pore size could be a suitable scaffold for endothelial cells and might be a promising implantable biomaterial with enhanced angiogenic capabilities for future clinical applications.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

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