In vitro study and pharmacokinetic evaluation of sitagliptin phosphate enantiomers in rat plasma

Author:

Spandana Tatineni1ORCID,Goli Veera VN1ORCID,Gurupadayya Bannimath1ORCID

Affiliation:

1. Department of Pharmaceutical Chemistry, JSS Academy of Higher Education & Research, Mysore, 570015, India

Abstract

Background: A chiral HPLC technique was developed to determine sitagliptin phosphate enantiomers in rat plasma in compliance with US FDA regulations. Methods & results: The technique used a Phenomenex column with a mobile phase consisting of a 60:35:5 (v/v/v) blend of pH4, 10-mM ammonium acetate buffer, methanol and 0.1% formic acid in Millipore water. The precision for both (R) and (S) sitagliptin phosphate varied between 0.246 and 1.246%, while the accuracy was 99.6–100.1%. A glucose uptake assay was used to assess enantiomers in 3T3-L1 cell lines through flow cytometry. Conclusion: Investigation of the pharmacokinetic impacts of sitagliptin phosphate racemic enantiomers in rat plasma revealed notable contrasts in R and S enantiomers in female albino Wistar rats, suggesting enantioselectivity for sitagliptin phosphate.

Funder

Department of Science and Technology, Ministry of Science and Technology, India

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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