Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research
Author:
Coleman-Gosser Nikki1,
Hu Yanhui2,
Raghuvanshi Shiva1,
Stitzinger Shane1,
Chen Weihang2,
Luhur Arthur3,
Mariyappa Daniel3ORCID,
Josifov Molly1ORCID,
Zelhof Andrew3,
Mohr Stephanie E2ORCID,
Perrimon Norbert24ORCID,
Simcox Amanda15ORCID
Affiliation:
1. Department of Molecular Genetics, Ohio State University
2. Drosophila RNAi Screening Center and Department of Genetics, Harvard Medical School
3. Drosophila Genomics Resource Center and Department of Biology, Indiana University
4. Howard Hughes Medical Institute
5. National Science Foundation
Abstract
Expression of activated Ras, RasV12, provides Drosophila cultured cells with a proliferation and survival advantage that simplifies the generation of continuous cell lines. Here, we used lineage-restricted RasV12 expression to generate continuous cell lines of muscle, glial, and epithelial cell type. Additionally, cell lines with neuronal and hemocyte characteristics were isolated by cloning from cell cultures established with broad RasV12 expression. Differentiation with the hormone ecdysone caused maturation of cells from mesoderm lines into active muscle tissue and enhanced dendritic features in neuronal-like lines. Transcriptome analysis showed expression of key cell-type-specific genes and the expected alignment with single-cell sequencing and in situ data. Overall, the technique has produced in vitro cell models with characteristics of glia, epithelium, muscle, nerve, and hemocyte. The cells and associated data are available from the Drosophila Genomic Resource Center.
Funder
National Institutes of Health (NIH) Office of the Director
National Institutes of Health
National Science Foundation
Howard Hughes Medical Institute
Women & Philanthropy at The Ohio State University
Publisher
eLife Sciences Publications, Ltd
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience