Odd-paired is a pioneer-like factor that coordinates with Zelda to control gene expression in embryos

Author:

Koromila Theodora1ORCID,Gao Fan1,Iwasaki Yasuno2,He Peng1ORCID,Pachter Lior1,Gergen J Peter2,Stathopoulos Angelike1ORCID

Affiliation:

1. California Institute of Technology, Division of Biology and Biological Engineering, Pasadena, United States

2. Stony Brook University, Department of Biochemistry and Cell Biology and Center for Developmental Genetics, Stony Brook, United States

Abstract

Pioneer factors such as Zelda (Zld) help initiate zygotic transcription inDrosophilaearly embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental patterns along the anterior-posterior axis. Finding that Opa also regulates expression through enhancersog_Distalalong the dorso-ventral axis, we hypothesized Opa’s role is more general. Chromatin-immunoprecipitation (ChIP-seq) confirmed its in vivo binding tosog_Distalbut also identified widespread binding throughout the genome, comparable to Zld. Furthermore, chromatin assays (ATAC-seq) demonstrate that Opa, like Zld, influences chromatin accessibility genome-wide at cellularization, suggesting both are pioneer factors with common as well as distinct targets. Lastly, embryos lackingopaexhibit widespread, late patterning defects spanning both axes. Collectively, these data suggest Opa is a general timing factor and likely late-acting pioneer factor that drives a secondary wave of zygotic gene expression.

Funder

National Institute of General Medical Sciences

Eunice Kennedy Shriver National Institute of Child Health and Human Development

Bioinformatics Resource Center at the Beckman Institute of Caltech

Stony Brook University College of Arts and Sciences

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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