Mitochondrial temperature homeostasis resists external metabolic stresses

Author:

Terzioglu Mügen1,Veeroja Kristo1,Montonen Toni1,Ihalainen Teemu O1,Salminen Tiina S1ORCID,Bénit Paule2,Rustin Pierre2ORCID,Chang Young-Tae3,Nagai Takeharu4,Jacobs Howard T15ORCID

Affiliation:

1. Faculty of Medicine and Health Technology, Tampere University

2. Université Paris Cité, Inserm, Maladies Neurodéveloppementales et Neurovasculaires

3. SANKEN (The Institute of Scientific and Industrial Research), Osaka University

4. Department of Chemistry, POSTECH

5. Department of Environment and Genetics, La Trobe University

Abstract

Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one insect cell line was estimated to be up to 15°C above that of the external environment to which the cells were exposed. High mitochondrial temperature was maintained in the face of a variety of metabolic stresses, including substrate starvation or modification, decreased ATP demand due to inhibition of cytosolic protein synthesis, inhibition of the mitochondrial adenine nucleotide transporter and, if an auxiliary pathway for electron transfer was available via the alternative oxidase, even respiratory poisons acting downstream of oxidative phosphorylation (OXPHOS) complex I. We propose that the high temperature of active mitochondria is an inescapable consequence of the biochemistry of OXPHOS and is homeostatically maintained as a primary feature of mitochondrial metabolism.

Funder

Academy of Finland

Basic Science Research Institute Fund

Sigrid Juséliuksen Säätiö

Japan Science and Technology Agency

National Research Foundation of Korea

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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