Validated HPLC and HPTLC Methods for Quantitative analysis of Syringic acid in Calendula officinalis

Abstract

Calendula officinalis L. have been recognized as most widely explored medicinal plant which have several pharmacological activities. The flowers of the Calendula officinalis have been used for from ancient time as remedy as Choleretic, phytopharmaceuticals, anti-inflammatory, regenerating, expectorants etc. The several pharmacological activities have been reported form the Calendula officinalis. Several compounds have been identified from the Calendula officinalis extract. Syringic acid have been recognized as important marker compound in the plant. The aim of the present research work was to develop and validate the methods for the quantitative analysis of syringic acid from the Calendula officinalis through RP-HPLC and HPTLC methods. The precision and accuracy of methods were calculated as per ICH guidelines. The developed methods for syringic acid determination were validated. The syringic acid in extract of Calendula officinalis was found 5.36% and 5.22% w/w from HPLC and HPTLC methods respectively. The HPLC and HPTLC methods can be used to determine the authenticity, assay, dissolution studies, bioequivalence studies and routine analysis in pharmaceutical industries.