A Validated RP-HPLC Method for Quantitative Estimation of Guaifenesin in Bulk and Pharmaceutical Dosage Forms-Reference-Cited by-同舟云学术

A Validated RP-HPLC Method for Quantitative Estimation of Guaifenesin in Bulk and Pharmaceutical Dosage Forms

Published:2023-01-27 Issue: Volume: Page:111-114
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Vemireddy Swapna.¹,Kumaraswamy Gandla²,Jyothsna B.³,Bindu M.⁴

Affiliation:

1. Research Scholar, Chaitanya (Deemed to be University), Department of Pharmacy, Warangal, Telangana - 506001, India.

2. Department of Pharmaceutical Analysis, Chaitanya (Deemed to be University), Hanumakonda, Telangana - 506001, India.

3. Chilkur Balaji College of Pharmacy, Aziz Nagar, Rangareddy, Telangana, 500075, India.

4. Marri Laxman Reddy (MLR) Institute of Pharmacy, Dundigal (Mandal), Medchal (District), Hyderabad - 500043, Telangana.

Abstract

A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase high performance liquid chromatographic (HPLC) method for the estimation of Guaifenesin in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved by Cosmosil packed (5C18-MS-II, 4.6 internal diameter 250mm lengths) column, using mobile phase ratio of Acetonitrile: Phosphate buffer PH 5(70:30). The flow rate was 1mL/min and the detection wavelength was 260nm. The limit of detection (LOD) for guaifenesin was 0.23µg/ml and the limit of quantification (LOQ) for guaifenesin was 0.78µg/ml. This method was validated concerning linearity, accuracy, precision, specificity and robustness. The method was also found to be stability-indicating.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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