Detection of Disinfectant property of purified Amylopullulanase from Citrobacter freundii SW

Abstract

There are varieties of marketable detergents and disinfectants purchased; in order to choose an effective one; it is very necessary to ensure its decontamination proficiency. So that a screening of pullulanase production from many isolates of Citrobacter freundii have been done using dried water cabbage as inducer of pullulanase production in the culture medium.Pullulanase producing bacteria was isolated and strain SW was designated. Morphological and biochemical tests in addition theanalysis of 16s rRNA observed that the strain SW revealed a 100% sequence identity withsimilarity to that of Citrobacter freundii. The pullulanase was purified by using two-step purification procedure consisting of ammonium sulphate precipitation, ion exchange chromatography followed by gel filtration chromatography with a yield of 29.7%. The pullulanase has an important role in augmentation of disinfectant efficiency. Depending on the current results, the combination between pullulanase and commercial disinfectant resulted to increase the disinfectant activity to greater levels against fungi more than bacteria.