MULTIFRACTAL CHARACTERISTICS OF THE MITOCHONDRIAL BK CHANNEL ACTIVITY IN THE PRESENCE OF THE CHANNEL-ACTIVATING FLAVONOIDS

Author:

TRYBEK PAULINA1ORCID,MACHURA ŁUKASZ1ORCID,BEDNARCZYK PIOTR2ORCID,COJG URSZULA3,PODLEŚNY PATRYCJA3,SZUMAŁA KAMILA3,WAWRZKIEWICZ-JAŁOWIECKA AGATA4ORCID

Affiliation:

1. Institute of Physics, Faculty of Science and Technology, University of Silesia in Katowice, 75 Pułku Piechoty 1A, 41-500 Chorzow, Poland

2. Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences — SGGW, Warszawa 02-787, Poland

3. Faculty of Biomedical Engineering, Silesian University of Technology, Gliwice 44-100, Poland

4. Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, Gliwice 44-100, Poland

Abstract

Over the years, the (multi)fractal nature of signals describing the activity of ion channels has been observed both at the level of single-channel currents and the temporal scaling of the corresponding sojourns in conducting and non-conducting states. The recognized nonlinear and self-similar properties were interpreted regarding the underlying channel gating machinery. Nevertheless, the literature lacks in (multi)fractal description of the biochemical stimulation of ion channels. Therefore, in this work, we provide a multifractal description of the effects exerted by two different flavonoids — naringenin (Nar) and quercetin (Que) — being the regulators of the large-conductance voltage- and Ca[Formula: see text]-activated K[Formula: see text] channels from the inner mitochondrial membrane (mitoBK). Toward this aim, the focus-based multifractal detrended fluctuation analysis (MFDFA) was applied to the patch-clamp data obtained in the presence of Nar and Que in micromolar concentrations and the corresponding controls. Our results show that mitoBK channel activity has well-pronounced multifractal characteristics and long-range memory (measured by the generalized Hurst exponent) under biochemical stimulation by Nar and Que and in the absence of these substances. The spectral properties significantly change after the randomization of the analyzed series. Therefore, multifractality is an inherent feature of the mitoBK channel currents, which stems from their statistical distribution and temporal organization. Both flavonoids have similar structures and exert channel-activating effects, but they differently affect the parameters of the multifractal spectra of the corresponding patch-clamp signals. Coordination of Que leads to more prominent changes in signal complexity than Nar at membrane hyper- and depolarization. It suggests that this flavonoid has a stronger impact on the conformational dynamics of the mitoBK channels in comparison with naringenin.

Funder

Silesian University of Technology

National Science Center of Poland

Publisher

World Scientific Pub Co Pte Ltd

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3