Epigallocatechin Gallate Reduces Homocysteine-Caused Oxidative Damages through Modulation SIRT1/AMPK Pathway in Endothelial Cells

Author:

Pai Pei-Ying12,Chou Wan-Ching3,Chan Shih-Hung4,Wu Shu-Yih567,Chen Hsiu-I8,Li Chi-Wen9,Hsieh Pei-Ling10,Chu Pei-Ming10,Chen Yu-An11,Ou Hsiu-Chung7,Tsai Kun-Ling312

Affiliation:

1. School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan, ROC

2. Division of Cardiology, China Medical University Hospital, Taichung, Taiwan, ROC

3. Department of Physical Therapy, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC

4. Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC

5. Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University, Taichung, Taiwan, ROC

6. Department of Physical and Rehabilitation Medicine, Asia University Hospital, Taichung, Taiwan, ROC

7. Department of Physical Therapy, College of Medical and Health Science, Asia University, Taichung, Taiwan, ROC

8. Department of Physical Therapy, Hungkuang University, Taichung, Taiwan, ROC

9. Department of Post-Baccalaureate Veterinary Medicine, College of Medical and Health Science, Asia University, Taichung, Taiwan, ROC

10. Department of Anatomy, School of Medicine, China Medical University, Taichung, Taiwan, ROC

11. Department of Health and Leisure Management, Yuanpei University of Medical Technology, Hsinchu, Taiwan, ROC

12. Institute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC

Abstract

Elevated plasma concentration of total homocysteine is a pathological condition that causes vascular endothelial injury and subsequently leads to the progression of endothelial apoptosis in atherosclerosis. Epigallocatechin gallate (EGCG), a well-known anti-oxidant in green tea, has been reported with benefits on metabolic and cardiovascular diseases. This study aimed to explore that EGCG ameliorates homocysteine-induced endothelial cell apoptosis through enhancing the sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK) survival signaling pathway. Human umbilical endothelial cells were treated with homocysteine in the presence or absence of EGCG. We found that EGCG significantly increased the activities of SIRT1 and AMPK. EGCG diminished homocysteine-mediated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation by inhibiting protein kinase C activation as well as reactive oxygen species (ROS) generation and recovered the activity of the endogenous antioxidant enzyme, superoxidase dismutase (SOD). Besides, EGCG also restores homocysteine-mediated dephosphorylation of Akt and decreases endothelial NO synthase (eNOS) expression. Furthermore, EGCG ameliorates homocysteine-activated pro-apoptotic events. The present study shows that EGCG prevents homocysteine-induced endothelial cell apoptosis via enhancing SIRT1/AMPK as well as Akt/eNOS signaling pathways. Results from this study indicated that EGCG might have some benefits for hyperhomocysteinemia.

Publisher

World Scientific Pub Co Pte Lt

Subject

Complementary and alternative medicine,General Medicine

全球学者库

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"全球学者库"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前全球学者库共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-88110370

www.globalauthorid.com

TOP

Copyright © 2019-2021 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3