Affiliation:
1. Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan
Abstract
Recent studies have reported gene therapy as an alternative treatment for canine’s cancer. Fifty-three samples from canine patients were obtained and the expression of MHC classes I and II and CD-markers in tumour cells, subpopulations of tumour-infiltrating lymphocytes (TILs), peripheral blood lymphocytes (PBLs) through flow cytometry and tumour cell cytokines were examined through real-time reverse transcription polymerase chain reaction. These data were compared by using Biomarker Patterns Software. Mammary gland tumours (MGTs) showed low MHC I expression that can be correlated with tumour malignancy, with cut-off values of 72.58% (MGT, [Formula: see text]% and non-MGT, [Formula: see text]%). IL-8, showing a positive correlation with angiogenesis, expression in MGTs was significantly high and IL-12 expression in complex and tubulopapillary carcinomas were significantly lower than that in healthy mammary gland cells ([Formula: see text]). PBLs of dogs with MGTs had fewer T cells, particularly T helper cells, and B cells and more non-T, non-B cells, particularly malignant MGTs, than did PBLs of healthy dogs. Among all surface markers and cytokines detected, high CD3 TIL was significantly correlated with a more favourable prognosis. Cytokines, such as IL-8, -12, and -15, can be the indicators of therapeutic targets because of their specific expression patterns in specific MGT subtypes.
Funder
Ministry of Science and Technology, Taiwan
Council of Agriculture
Publisher
World Scientific Pub Co Pte Lt