A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.-Reference-Cited by-同舟云学术

A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

Published:1992-11 Issue:2 Volume:2 Page:144-148
ISSN:1088-9051
Container-title:Genome Research
language:en
Short-container-title:Genome Res.

Author:

Borson N D,Salo W L,Drewes L R

Abstract

We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.

Publisher

Cold Spring Harbor Laboratory

Subject

Genetics (clinical),Genetics

Reference24 articles.

1. Frohman, M.A. 1990. RACE: Rapid amplification of cDNA ends. In PCR protocols: A guide to methods and applications (eds. M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White), pp. 28–38. Academic, San Diego.

2. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

3. Mouse opsin: gene structure and molecular basis of multiple transcripts.;J. Biol. Chem.,1990

4. Rapid amplification of cDNA ends using nested primers.;Technique,1989

5. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries byin vitroamplification

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