Author:
Moukarzel Elsie,Antoine Sharmilee,Guinoiseau Sophie,Rubino Bruna,Stinnakre Jacques,Agulhon Cendra
Abstract
AbstractAstrocytes are the most abundant glial cells in the central nervous system and interact with other cell types, including neurons and microglia,viaGqprotein-coupled receptors (GqGPCRs) present on their surface. Astrocytic GqGPCR activation induces Ca2+release from internal stores, leading to intracellular Ca2+elevations. There is emerging evidence supporting that astrocytic GqGPCR Ca2+elevations are upregulated and dysregulated in neurodegenerative diseases and are thought to play an important role in the pathogenesis of such diseases. Furthermore, astrocytic GqGPCR Ca2+-dependent release of neuroactive or inflammatory molecules from astrocytes may occur in the early steps of the stress/inflammatory process in the diseased brain. In addition, low grade and chronic brain inflammation is involved in the etiology of neurodegenerative diseases.We hypothesized that chronic activation of astrocytic GqGPCR Ca2+signaling leads to an altered production of glutamate or pro-inflammatory factors from astrocytes, and consequent deficits in synaptic transmission, long-term potentiation (LTP), and memory formation. To test this hypothesis, we used an AAV-based chemogenetic tool to selectively activate astrocyte GqGPCR Ca2+signaling combined within vivoelectrophysiology, immunohistochemistry, and biochemistry.Using the mouse primary visual cortex (V1) as a model system, we found that chronically increased astrocytic GqGPCR Ca2+signaling leads to a decrease in LTP of visual-evoked potentials. Such LTP impairment was associated with microglial reactive phenotype - displaying a hyper-ramified and proliferative state - as well as a decrease in the number of interleukin 33 (IL-33)-expressing astrocytes. Our study is the first to have shown that chronic astrocytic GqGPCR activation is sufficient to alter visual LTP and induce astrocyte-to-microglia communication, possibly through and IL-33 pathway in the adult brain. Because GPCRs are important drug targets, our study could have relevant therapeutic implications in the treatment of some neurodegenerative diseases.
Publisher
Cold Spring Harbor Laboratory