Author:
Ulrich Nicole D,Shen Yu-chi,Ma Qianyi,Yang Kun,Hannum D Ford,Jones Andrea,Machlin Jordan,Randolph John F,Smith Yolanda R,Schon Samantha B,Shikanov Ariella,Marsh Erica E.,Li Jun Z,Hammoud Sue
Abstract
AbstractFallopian tube (FT) homeostasis requires dynamic regulation of heterogeneous cell populations and is disrupted in infertility and ovarian cancer. Here we applied single-cell RNAseq to profile 53,376 FT cells from 3 healthy pre-menopausal subjects. The resulting cell atlas contains 12 major cell types representing epithelial, stromal and immune compartments. Re-clustering of epithelial cells identified 4 ciliated and 6 non-ciliated secretory epithelial subtypes, two of which represent potential progenitor pools: one leading to mature secretory cells, while the other contributing to either ciliated cells or one of the stromal cell types. To understand how FT cell numbers and states change in a disease state, we analyzed ~15,000 cells from a hydrosalpinx sample and observed shifts in epithelial and stromal populations, and cell type-specific changes in extracellular matrix and TGF-β signaling, underscoring fibrosis pathophysiology. This resource is expected to facilitate future studies to understand fallopian tube homeostasis in normal development and disease.
Publisher
Cold Spring Harbor Laboratory
Reference37 articles.
1. Ajonuma, Louis Chukwuemeka , Ernest Hung Yu Ng , Lin Nga Chan , Pak Ham Chow , Lai Sin Kung , Annie Nga Yin Cheung , Lok Sze Ho , et al. 2005. “Ultrastructural Characterization of Whole Hydrosalpinx from Infertile Chinese Women.” Cell Biology International, 8.
2. Characterization of the Immune Cell Repertoire in the Normal Fallopian Tube;International Journal of Gynecological Pathology□: Official Journal of the International Society of Gynecological Pathologists,2014
3. Barton, Brooke E , Gerardo G Herrera , Prashanth Anamthathmakula , Jenna K Rock , Anna M Willie , Emily A Harris , Ken-Ichi Takemaru , and Wipawee Winuthayanon . 2021. “Roles of Steroid Hormones in Oviductal Function,” 25.
4. TGF-β signaling in fibrosis
5. Epithelial Stem Cells: Turning over New Leaves
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献