The Expression Pattern of miR-17, −24, −124 and −145 as Diagnostic Factor for Metastatic Gastric Cancer; a Lesson from Gastric Cancer Stem cells

Author:

Yasavoli-Sharahi Hamed,Jahangiri-Tazehkand Soheil,Iranmehr Zahra,Eslahchi Changiz,Razavi Amirnader Emami,Moradi Sharif,Asl Niloofar Shayan,Memari Fereidoon,Ebrahimi Marzieh

Abstract

AbstractBackgroundDistant metastasis of Gastric Cancer (GC) causes more than 700 000 deaths worldwide. Cancer Stem Cells (CSCs) are a subpopulation of cancer cells responsible for aggressiveness and chemoresistance in clinical settings. MicroRNAs (miRNAs) emerge as important players in regulating self-renewal and metastasis in CSCs. Understanding the role of miRNAs in CSCs offer a potential diagnostic tool for GC patients. This study is aimed to identify miRNAs that target both stemness and metastasis in gastric cancer stem cells (GCSCs) and differentially expressed in metastatic GC patients as diagnostic biomarkers for GC metastasis.MethodsWe investigate the gene expression profile of patients using the GEO database and Rstudio software. To obtain the regulatory networks and miRNAs, the STRING and miRwalk database used. The gastric cancer tissues were obtained from Iranian National Tumor Bank (INTB) to validate the results.ResultsOur results indicated three important regulatory cores affecting the immune system’s regulation, tumor progress, and metastasis. Based on the bioinformatics results, four miRNAs miR-17-5p, miR-24-3p, miR-124-3p, and miR-145-5p, were selected, and their expression pattern was evaluated in 10 patients’ metastatic tumors compared to 10 nonmetastatic tumors by real-time PCR. The expression level of mir-17, −24, and −124 was upregulated about 8, 10, 60 folds, respectively, and miR-145 was down-regulated 4.5 folds in metastatic tumors compared to nonmetastatic tumors.Conclusionthe high expression level of miR-17, −24, −124, and low level of miR-145 in GC patients’ samples could be a potential biomarker for the presence of GCSCs and the diagnosis of metastasis.

Publisher

Cold Spring Harbor Laboratory

Reference87 articles.

1. (GCO), G.C.O. Gastric cancer incidence. 2020; Available from: https://gco.iarc.fr/today/data/factsheets/cancers/7-Stomach-fact-sheet.pdf.

2. Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray;World journal of gastroenterology: WJG,2006

3. Common stemness regulators of embryonic and cancer stem cells;World journal of stem cells,2015

4. Expression profile of cytokines in gastric cancer patients using proteomic antibody microarray;Oncology letters,2017

5. Lysine methylation of transcription factors in cancer

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3